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ATCC reference strain e coli atcc 25922
Reference Strain E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+atcc+25922+reference+strains/Klebsiella+quasipneumoniae+Brisse+et+al/pm42304017-155-6-10
Average 99 stars, based on 5101 article reviews

reference strain e coli atcc 25922 - by Bioz Stars, 2026-09

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Activity Assay:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. Antibacterial activity of various concentrations of TAuNPs and NCS-TAuNPs in a solution of 1% DMSO, i.e., 50 μg/mL, 100 μg/mL, and 150 μg/mL, was explored against two bacteria, which are present in human urinary tracts and are resistant to the majority of drugs, i.e., ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains.. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gramnegative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS – TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/ mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gram-negative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS –TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Bacteria:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. Antibacterial activity of various concentrations of TAuNPs and NCS-TAuNPs in a solution of 1% DMSO, i.e., 50 μg/mL, 100 μg/mL, and 150 μg/mL, was explored against two bacteria, which are present in human urinary tracts and are resistant to the majority of drugs, i.e., ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains.. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gramnegative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS – TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/ mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gram-negative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS –TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Infection:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gramnegative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS – TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/ mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gram-negative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS –TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/mL) of nanoparticles were prepared in 1 mL of DMSO. ..

In Vitro:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gramnegative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS – TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/ mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gram-negative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS –TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Diffusion-based Assay:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gramnegative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS – TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/ mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: .. Antibacterial activity of TAuNPs and NCS –TAuNPs conjugate against two urinary tract infection Gram-negative bacteria isolates; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains were assessed in vitro using different concentrations of TAuNPs and NCS –TAuNPs composites by agar diffusion methods according to US CLSI (Clinical and Laboratory Standards Institute) protocol, The TAuNPs and NCS-TAuNPs were blended with 0.5% dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and various concentrations (50, 100, and 150 μg/mL) of nanoparticles were prepared in 1 mL of DMSO. ..

Control:

Article Title: Integrated analysis of global regulators and efflux genes associated with antimicrobial resistance reversal in multidrug resistant Klebsiella pneumoniae.
Article Snippet: .. Quality control verification was performed in every MIC run using K. pneumoniae ATCC 13883 and E. coli ATCC 25922 reference strains to ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS ensure that susceptibility values fell within CLSI-accepted QC ranges. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: Overall, the reverse transcriptase analysis revealed a significant decrease in the expression profiles of virulence genes ( entB-F , fimH , pgaA , blaKPC, blaNDM1 , blaCTX-M1 and luxS ) in multi-drug resistant biofilm-forming of uropathogenic strains; K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains biofilm treated with 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h compared with 10 μg/mL gentamycin as a positive control and negative control (0.9% NaCl) groups (Fig. ). .. A hierarchical clustering heatmap of the transcription of the genes investigated is shown in Fig. . Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. A) showed that the minimum expression level (highest antibiofilm efficiency) 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1 , blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. S5A) showed that the minimum expression level (highest antibiofilm efficiency) Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1, blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

Crystal Violet Assay:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. After mature biofilm formation of in multi-drug resistant biofilm-forming of uropathogenic strains; K. pneumoniae ESA254 and E. coli ESA253, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains and detected by crystal violet assay, The anti-biofilm efficiency of different concentrations (50, 100, and 150 μg/ ml) of TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO against the investigated K. pneumoniae ESA254 and E. coli ESA253 strains and TAuNPs and NCS–TAuNPs are significantly reduced the amount of preformed biofilm in the total population (CFU/mL) of K. pneumoniae ESA254 and E. coli ESA253 in a dosedependent manner. ..

Expressing:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: Overall, the reverse transcriptase analysis revealed a significant decrease in the expression profiles of virulence genes ( entB-F , fimH , pgaA , blaKPC, blaNDM1 , blaCTX-M1 and luxS ) in multi-drug resistant biofilm-forming of uropathogenic strains; K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains biofilm treated with 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h compared with 10 μg/mL gentamycin as a positive control and negative control (0.9% NaCl) groups (Fig. ). .. A hierarchical clustering heatmap of the transcription of the genes investigated is shown in Fig. . Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. A) showed that the minimum expression level (highest antibiofilm efficiency) 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1 , blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: And the highest anti-biofilm efficiency (biofilm formation reduction) in E. coli ATCC 25922 reference strain (79.25 ± 5.23%, 71.61 ± 4.1%, and 56.3 ± 2.9% were observed when K. pneumoniae biofilm was treated with the NCS–TAuNPs conjugate at 150 μg/mL, 100 μg/ mL, and 50 μg/mL, respectively. .. In contrast, the lowest Table 3 Biofilm reduction percentage (%) of UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains obtained after 24 h of exposure to the various concentrations (50 μg/ mL, 100 μg/mL and 150 μg/mL) of TAuNPs and NCS –TAuNPs conjugate, as compared with 10 μg/mL Gentamycin as a positive control and negative control (0.9% NaCl) groups, (value, N = 6) Treatments (μg/ ml) Anti-biofilm reduction (%) K.pneumoniae ESA254 E.coli ESA253 K.pneumoniae ATCC13883 E. coli ATCC TAuNPs 50 40.54 ± 2.15 36.55 ± 1.56 34.24 ± 2.1 43.7 ± 2.64 100 47.16 ± 3.07 42.9 ± 2.38 43.78 ± 2.56 51.20 ± 3.05 150 63.82 ± 1.89 58.07 ± 2.13 52.17 ± 4.02 63.4 ± 2.7 NCS– TAuNPs 50 68.11 ± 2.42 62.48 ± 1.78 60.73 ± 2.44 56.3 ± 2.9 100 73.46 ± 3.71 69.27 ± 3.40 70.6 ± 2.6 71.61 ± 4.1 150 85.67 ± 2.46 77.50 ± 2.8 81.06 ± 5.21 79.25 ± 5.23 gentamycin 10 94.02 ± 3.12 92.68 ± 3.05 93.19 ± 4.62 91.58 ± 5.7 Expression levels of Virulence Genes of K. pneumoniae and E. coli strains anti-biofilm efficiency (43.7 ± 2.64% and 51.20 ± 3.05%) were recorded at 50 μg/mL and 100 μg/mL of TAuNPs, respectively. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. S5A) showed that the minimum expression level (highest antibiofilm efficiency) Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1, blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

Positive Control:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: Overall, the reverse transcriptase analysis revealed a significant decrease in the expression profiles of virulence genes ( entB-F , fimH , pgaA , blaKPC, blaNDM1 , blaCTX-M1 and luxS ) in multi-drug resistant biofilm-forming of uropathogenic strains; K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains biofilm treated with 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h compared with 10 μg/mL gentamycin as a positive control and negative control (0.9% NaCl) groups (Fig. ). .. A hierarchical clustering heatmap of the transcription of the genes investigated is shown in Fig. . Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. A) showed that the minimum expression level (highest antibiofilm efficiency) 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1 , blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: And the highest anti-biofilm efficiency (biofilm formation reduction) in E. coli ATCC 25922 reference strain (79.25 ± 5.23%, 71.61 ± 4.1%, and 56.3 ± 2.9% were observed when K. pneumoniae biofilm was treated with the NCS–TAuNPs conjugate at 150 μg/mL, 100 μg/ mL, and 50 μg/mL, respectively. .. In contrast, the lowest Table 3 Biofilm reduction percentage (%) of UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains obtained after 24 h of exposure to the various concentrations (50 μg/ mL, 100 μg/mL and 150 μg/mL) of TAuNPs and NCS –TAuNPs conjugate, as compared with 10 μg/mL Gentamycin as a positive control and negative control (0.9% NaCl) groups, (value, N = 6) Treatments (μg/ ml) Anti-biofilm reduction (%) K.pneumoniae ESA254 E.coli ESA253 K.pneumoniae ATCC13883 E. coli ATCC TAuNPs 50 40.54 ± 2.15 36.55 ± 1.56 34.24 ± 2.1 43.7 ± 2.64 100 47.16 ± 3.07 42.9 ± 2.38 43.78 ± 2.56 51.20 ± 3.05 150 63.82 ± 1.89 58.07 ± 2.13 52.17 ± 4.02 63.4 ± 2.7 NCS– TAuNPs 50 68.11 ± 2.42 62.48 ± 1.78 60.73 ± 2.44 56.3 ± 2.9 100 73.46 ± 3.71 69.27 ± 3.40 70.6 ± 2.6 71.61 ± 4.1 150 85.67 ± 2.46 77.50 ± 2.8 81.06 ± 5.21 79.25 ± 5.23 gentamycin 10 94.02 ± 3.12 92.68 ± 3.05 93.19 ± 4.62 91.58 ± 5.7 Expression levels of Virulence Genes of K. pneumoniae and E. coli strains anti-biofilm efficiency (43.7 ± 2.64% and 51.20 ± 3.05%) were recorded at 50 μg/mL and 100 μg/mL of TAuNPs, respectively. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. S5A) showed that the minimum expression level (highest antibiofilm efficiency) Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1, blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

Negative Control:

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells
Article Snippet: Overall, the reverse transcriptase analysis revealed a significant decrease in the expression profiles of virulence genes ( entB-F , fimH , pgaA , blaKPC, blaNDM1 , blaCTX-M1 and luxS ) in multi-drug resistant biofilm-forming of uropathogenic strains; K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains biofilm treated with 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h compared with 10 μg/mL gentamycin as a positive control and negative control (0.9% NaCl) groups (Fig. ). .. A hierarchical clustering heatmap of the transcription of the genes investigated is shown in Fig. . Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. A) showed that the minimum expression level (highest antibiofilm efficiency) 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1 , blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: And the highest anti-biofilm efficiency (biofilm formation reduction) in E. coli ATCC 25922 reference strain (79.25 ± 5.23%, 71.61 ± 4.1%, and 56.3 ± 2.9% were observed when K. pneumoniae biofilm was treated with the NCS–TAuNPs conjugate at 150 μg/mL, 100 μg/ mL, and 50 μg/mL, respectively. .. In contrast, the lowest Table 3 Biofilm reduction percentage (%) of UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains obtained after 24 h of exposure to the various concentrations (50 μg/ mL, 100 μg/mL and 150 μg/mL) of TAuNPs and NCS –TAuNPs conjugate, as compared with 10 μg/mL Gentamycin as a positive control and negative control (0.9% NaCl) groups, (value, N = 6) Treatments (μg/ ml) Anti-biofilm reduction (%) K.pneumoniae ESA254 E.coli ESA253 K.pneumoniae ATCC13883 E. coli ATCC TAuNPs 50 40.54 ± 2.15 36.55 ± 1.56 34.24 ± 2.1 43.7 ± 2.64 100 47.16 ± 3.07 42.9 ± 2.38 43.78 ± 2.56 51.20 ± 3.05 150 63.82 ± 1.89 58.07 ± 2.13 52.17 ± 4.02 63.4 ± 2.7 NCS– TAuNPs 50 68.11 ± 2.42 62.48 ± 1.78 60.73 ± 2.44 56.3 ± 2.9 100 73.46 ± 3.71 69.27 ± 3.40 70.6 ± 2.6 71.61 ± 4.1 150 85.67 ± 2.46 77.50 ± 2.8 81.06 ± 5.21 79.25 ± 5.23 gentamycin 10 94.02 ± 3.12 92.68 ± 3.05 93.19 ± 4.62 91.58 ± 5.7 Expression levels of Virulence Genes of K. pneumoniae and E. coli strains anti-biofilm efficiency (43.7 ± 2.64% and 51.20 ± 3.05%) were recorded at 50 μg/mL and 100 μg/mL of TAuNPs, respectively. ..

Article Title: Antimicrobial and anti-cancer potential of turmeric synthesized AuNPs and Chitosan-AuNP nanocomposites against MDR pathogens and breast/colorectal carcinoma cells.
Article Snippet: .. The expression profile of virulent genes in K. pneumoniae ESA254 strain exposed to 150 μg/ml TAuNPs and NCS –TAuNPs conjugate in a 1.0% DMSO solution for 24 h (Supplementary Fig. S5A) showed that the minimum expression level (highest antibiofilm efficiency) Fig. 5 Hierarchical clustering heatmap of the transcription of relative expression of entA-F, fimH, pgaA, blaKPC, blaNDM, blaCTX-M and luxS resistance and virulent genes in UTIs K. pneumoniae ESA254 and E. coli ESA253 isolates, and K. pneumoniae ATCC13883 and E. coli ATCC 25922 reference strains exposed to 150 μg/ml TAuNPs and NCS–TAuNPs conjugate dissolved in 1.0% DMSO for 24 h 0.25 and 0.33 fold change of fimH virulence gene exposed to 150 μg/ml NCS –TAuNPs conjugate and TAuNPs nanoparticles respectively as compared with 0.2 fold change expression level in K. pneumoniae ESA254 isolate biofilm exposed to 10 μg/mL gentamycin positive control and 0.98 fold change in untreated K. pneumoniae biofilm control, followed with 0.37, 0.4, 0.44, 0.57and 0.67 and 0.74 fold changes of blaNDM1, blaCTX-M, entB-F, luxS, and pgaA resistance genes in K. pneumoniae biofilm exposed to 150 μg/ml NCS –TAuNPs conjugate respectively, while the maximum expression level and (lowest antibiofilm efficiency) 0.87 fold change of blaKPC virulent gene in K. pneumoniae biofilm exposed to 100 μg/ml TAuNPs was observed compared with positive and negative control. ..

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